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1.
J Endod ; 45(2): 116-122, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30711166

RESUMO

INTRODUCTION: The aim of this study was to determine the prognostic factors of periapical healing of teeth after single-visit nonsurgical endodontic treatment under general anesthesia in special needs patients. METHODS: This retrospective study collected 448 teeth from 241 patients (mean age = 27.4 years, standard deviation = 13.9 years). A total of 271 teeth (60.5%) with clinical and radiographic follow-up records longer than 12 months (mean [standard deviation] = 56.1 [27.9]) were included in the outcome analysis. Demographic, systemic, and dental information about the cases were compared between the healed and nonhealed groups using the Pearson chi-square test. Factors contributing to periapical healing were determined with multivariate logistic regression analysis. RESULTS: Complete periapical healing without clinical signs was observed in 221 teeth (81.5%), uncertain healing with decreased size of the periapical lesion was shown in 43 teeth (15.9%), and no reduction in lesion size was observed in 7 cases (2.6%). In the multivariate analysis, diet type, oral hygiene maintenance, pulp vitality, and root filling length were detected as contributing factors for complete resolution of periapical lesions (P < .05). CONCLUSIONS: Successful outcomes of single-visit endodontic treatment under general anesthesia were shown in special needs patients. The prognoses were influenced by the clinical circumstances of the patients and the preoperative conditions of their teeth.


Assuntos
Anestesia Geral , Transtornos da Comunicação , Deficiência Intelectual , Doenças Periapicais/fisiopatologia , Doenças Periapicais/cirurgia , Tratamento do Canal Radicular , Ápice Dentário/fisiopatologia , Cicatrização , Adolescente , Adulto , Dieta , Feminino , Seguimentos , Humanos , Masculino , Higiene Bucal , Prognóstico , Estudos Retrospectivos , Inquéritos e Questionários , Fatores de Tempo , Adulto Jovem
2.
Braz Oral Res ; 30(1)2016 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-27556552

RESUMO

Revascularization of immature teeth with necrotic pulps traditionally involves the use of triple antibiotic paste, which may sometimes lead to undesirable complications. The objective of this study was to assess tissue repair in immature dog teeth with apical periodontitis subjected to revascularization, comparing two different pastes used for root canal disinfection. Apical periodontitis was induced in 30 dog premolars. Teeth were randomly divided into three experimental groups: root canals filled with triple antibiotic paste (n = 10); root canals filled with 1% propolis paste (n = 10); and no medication (n = 10). An additional group (n = 10, no intervention) was used as control. After 7 months, the jaws were histologically evaluated for the following variables: newly formed mineralized tissue (present/absent); vital tissue in the canal space (absent/periodontal ligament-like/pulp-like); apical extension of root (present/absent); and severity of inflammatory process (absent/mild/moderate/severe). There were no statistically significant differences among the experimental groups in new mineralized tissue formation and apical root development. The formation of vital tissue in the canal space, in turn, was statistically different between the triple paste and propolis groups: vital tissues were present in all revascularized teeth disinfected with propolis paste (100%), compared to 71% of those disinfected with the triple paste. Severity of inflammatory process was different between the triple paste and no medication groups. The new tissues formed onto canal walls and in the root canal space showed characteristics of cementum and periodontal ligament, respectively. Propolis may have some advantages over the triple paste for the revascularization of immature teeth.


Assuntos
Anti-Infecciosos/farmacologia , Necrose da Polpa Dentária/tratamento farmacológico , Regeneração Tecidual Guiada/métodos , Neovascularização Fisiológica/efeitos dos fármacos , Periodontite Periapical/tratamento farmacológico , Própole/farmacologia , Irrigantes do Canal Radicular/farmacologia , Dente/irrigação sanguínea , Animais , Anti-Infecciosos/uso terapêutico , Polpa Dentária/efeitos dos fármacos , Polpa Dentária/fisiopatologia , Cavidade Pulpar/efeitos dos fármacos , Cavidade Pulpar/fisiopatologia , Necrose da Polpa Dentária/fisiopatologia , Dentina/efeitos dos fármacos , Cães , Pomadas , Periodontite Periapical/fisiopatologia , Ligamento Periodontal/efeitos dos fármacos , Própole/uso terapêutico , Distribuição Aleatória , Reprodutibilidade dos Testes , Irrigantes do Canal Radicular/uso terapêutico , Fatores de Tempo , Ápice Dentário/efeitos dos fármacos , Ápice Dentário/fisiopatologia , Remineralização Dentária/métodos , Resultado do Tratamento
3.
Aust Dent J ; 61 Suppl 1: 95-106, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26923451

RESUMO

Endodontic management of the permanent immature tooth continues to be a challenge for both clinicians and researchers. Clinical concerns are primarily related to achieving adequate levels of disinfection as 'aggressive' instrumentation is contraindicated and hence there exists a much greater reliance on endodontic irrigants and medicaments. The open apex has also presented obturation difficulties, notably in controlling length. Long-term apexification procedures with calcium hydroxide have proven to be successful in retaining many of these immature infected teeth but due to their thin dentinal walls and perceived problems associated with long-term placement of calcium hydroxide, they have been found to be prone to cervical fracture and subsequent tooth loss. In recent years there has developed an increasing interest in the possibility of 'regenerating' pulp tissue in an infected immature tooth. It is apparent that although the philosophy and hope of 'regeneration' is commendable, recent histologic studies appear to suggest that the calcified material deposited on the canal wall is bone/cementum rather than dentine, hence the absence of pulp tissue with or without an odontoblast layer.


Assuntos
Apexificação/métodos , Ápice Dentário/fisiopatologia , Traumatismos Dentários/complicações , Compostos de Alumínio/uso terapêutico , Compostos de Cálcio/uso terapêutico , Hidróxido de Cálcio/uso terapêutico , Combinação de Medicamentos , Humanos , Odontogênese/fisiologia , Óxidos/uso terapêutico , Pulpotomia/métodos , Regeneração/fisiologia , Irrigantes do Canal Radicular/uso terapêutico , Silicatos/uso terapêutico , Ápice Dentário/patologia , Traumatismos Dentários/fisiopatologia
4.
Aust Dent J ; 61 Suppl 1: 120-7, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26923453

RESUMO

Life cycles of dental trauma victims can provide important clinical information, especially when viewed over many years. In this first series of life cycles, the pulp and periodontal responses to traumatic injuries of four patients are documented over periods varying from 26 to 51 years. The dynamics of pulp survival following an intrusive luxation and two avulsions are followed, with particular reference to pulp canal calcification to which a new term, root canal stenosis, has been proposed. The life cycles include the successful management of inflammatory root resorption in a replanted tooth with an open apex contrasting with the early prophylactic endodontic treatment of two replanted teeth in a patient with mature apices. The long-term development of invasive cervical resorption in one of the patient's life cycle highlights the importance of ongoing follow-up examinations for dental trauma victims.


Assuntos
Traumatismos Dentários/complicações , Adolescente , Criança , Estudos de Coortes , Constrição Patológica/etiologia , Polpa Dentária/fisiopatologia , Calcificações da Polpa Dentária/etiologia , Cavidade Pulpar/fisiopatologia , Necrose da Polpa Dentária/etiologia , Feminino , Seguimentos , Humanos , Incisivo/lesões , Estudos Longitudinais , Masculino , Odontogênese/fisiologia , Periodonto/fisiopatologia , Tratamento do Canal Radicular/métodos , Reabsorção da Raiz/etiologia , Ápice Dentário/fisiopatologia , Avulsão Dentária/complicações , Avulsão Dentária/fisiopatologia , Colo do Dente/fisiopatologia , Traumatismos Dentários/fisiopatologia , Reimplante Dentário/métodos , Adulto Jovem
5.
J Endod ; 42(1): 150-5, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26518216

RESUMO

INTRODUCTION: Endodontic-periodontal (EP) lesions require both endodontic and periodontal therapies. Impermeable sealing of the root canal system after cleaning and shaping is essential for a successful endodontic treatment. However, complete healing of the hard and soft tissue lesions takes time, and diseased bone, periodontal ligament, and tooth fibrous joints are reported to have an increased failure risk for a given load. Considering that EP lesions may affect the biomechanics of teeth, this finite elemental analysis study aimed to test the effect of root fillings on stress distribution in premolars with EP lesions. METHODS: Three finite elemental analysis models representing 3 different types of EP lesions (primary endodontic disease [PED], PED with secondary periodontic involvement, and true combined) were created. The root canals were assumed as nonfilled or filled with gutta-percha, gutta-percha/apical mineral trioxide aggregate (MTA) plug, and MTA-based sealer. Materials used were assumed to be homogenous and isotropic. A 300-N load was applied from the buccal cusp of the crown with a 135° angle. The Cosmoworks structural-analysis program (SolidWorks Corp, Waltham, MA) was used for analysis. Results were presented considering von Mises criteria. RESULTS: Stresses at the root apex increased with an increase in lesion dimensions. Root filling did not affect stress distribution in the PED model. An MTA plug or MTA-based sealer created more stress areas within the root compared with the others in the models representing PED with periodontic involvement and true combined lesions. CONCLUSIONS: Stresses at the apical end of the root increase with increases in lesion dimensions. MTA-based sealers or an MTA plug creates more stresses when there is periodontic involvement or a true combined lesion.


Assuntos
Dente Pré-Molar/fisiopatologia , Doenças Periodontais/fisiopatologia , Materiais Restauradores do Canal Radicular/efeitos adversos , Ápice Dentário/fisiopatologia , Compostos de Alumínio/efeitos adversos , Compostos de Cálcio/efeitos adversos , Combinação de Medicamentos , Análise de Elementos Finitos , Guta-Percha/efeitos adversos , Humanos , Óxidos/efeitos adversos , Silicatos/efeitos adversos , Estresse Mecânico
6.
Braz. oral res. (Online) ; 30(1): e74, 2016. tab, graf
Artigo em Inglês | LILACS | ID: biblio-952057

RESUMO

Abstract Revascularization of immature teeth with necrotic pulps traditionally involves the use of triple antibiotic paste, which may sometimes lead to undesirable complications. The objective of this study was to assess tissue repair in immature dog teeth with apical periodontitis subjected to revascularization, comparing two different pastes used for root canal disinfection. Apical periodontitis was induced in 30 dog premolars. Teeth were randomly divided into three experimental groups: root canals filled with triple antibiotic paste (n = 10); root canals filled with 1% propolis paste (n = 10); and no medication (n = 10). An additional group (n = 10, no intervention) was used as control. After 7 months, the jaws were histologically evaluated for the following variables: newly formed mineralized tissue (present/absent); vital tissue in the canal space (absent/periodontal ligament-like/pulp-like); apical extension of root (present/absent); and severity of inflammatory process (absent/mild/moderate/severe). There were no statistically significant differences among the experimental groups in new mineralized tissue formation and apical root development. The formation of vital tissue in the canal space, in turn, was statistically different between the triple paste and propolis groups: vital tissues were present in all revascularized teeth disinfected with propolis paste (100%), compared to 71% of those disinfected with the triple paste. Severity of inflammatory process was different between the triple paste and no medication groups. The new tissues formed onto canal walls and in the root canal space showed characteristics of cementum and periodontal ligament, respectively. Propolis may have some advantages over the triple paste for the revascularization of immature teeth.


Assuntos
Animais , Cães , Periodontite Periapical/tratamento farmacológico , Própole/farmacologia , Irrigantes do Canal Radicular/farmacologia , Dente/irrigação sanguínea , Neovascularização Fisiológica/efeitos dos fármacos , Necrose da Polpa Dentária/tratamento farmacológico , Regeneração Tecidual Guiada/métodos , Anti-Infecciosos/farmacologia , Pomadas , Periodontite Periapical/fisiopatologia , Ligamento Periodontal/efeitos dos fármacos , Própole/uso terapêutico , Irrigantes do Canal Radicular/uso terapêutico , Fatores de Tempo , Remineralização Dentária/métodos , Distribuição Aleatória , Reprodutibilidade dos Testes , Resultado do Tratamento , Necrose da Polpa Dentária/fisiopatologia , Ápice Dentário/efeitos dos fármacos , Ápice Dentário/fisiopatologia , Polpa Dentária/efeitos dos fármacos , Polpa Dentária/fisiopatologia , Cavidade Pulpar/efeitos dos fármacos , Cavidade Pulpar/fisiopatologia , Dentina/efeitos dos fármacos , Anti-Infecciosos/uso terapêutico
7.
J Endod ; 41(3): 412-6, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25576200

RESUMO

INTRODUCTION: In a clinical situation, an apically resected tooth is often accompanied by a varying degree of periodontal bone loss. The purpose of this study was to assess the influence of apical root resection combined with periodontal bone loss on the biomechanical response of a single-rooted tooth. METHODS: A basic intact model and a basic apically resected model of the upper central incisor were selected for the numerical analysis. From each basic model, 6 models were developed assuming different amounts of periodontal bone loss (0, 0.5, 1, 1.5, 2, and 3 mm). Maximum von Mises stress (σ max), maximum tooth displacement (ΔR max), and effective crown-to-root ratio (α) were calculated for each condition. RESULTS: There were only marginal differences (a 2.1% difference in σ max and a 16.9% difference in ΔR max) between the biomechanical responses of the intact model and the apically resected model when the tooth was supported by a normal periodontium. However, when destruction of the periodontium was assumed, the intact model and the apically resected model responded differently. The difference increased as the periodontal bone loss progressed, resulting in a 68.7% difference in σ max and a 56.3% difference in ΔR max when the periodontal bone loss increased to 3 mm (α = 0.48). CONCLUSIONS: Although the biomechanical response of an apically resected tooth was relatively stable when the tooth was supported by a normal periodontium, the apically resected tooth showed a more deteriorated response compared with the intact tooth as the periodontal bone loss progressed.


Assuntos
Perda do Osso Alveolar/fisiopatologia , Ápice Dentário/fisiopatologia , Ápice Dentário/cirurgia , Fenômenos Biomecânicos , Análise do Estresse Dentário , Análise de Elementos Finitos , Humanos , Modelos Biológicos , Estresse Mecânico , Ápice Dentário/patologia , Coroa do Dente/patologia , Coroa do Dente/fisiopatologia
8.
J Endod ; 41(2): 190-6, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25498834

RESUMO

INTRODUCTION: The purpose of this study was to present a new periapical and endodontic status scale (PESS) that is based on the complex periapical index (COPI), which was designed for the identification and classification of periapical bone lesions in cases of apical periodontitis, and the endodontically treated tooth index, which was designed for endodontic treatment quality evaluation by means of cone-beam computed tomographic (CBCT) analysis. METHODS: Periapical and endodontic status parameters were selected from the already known indexes and scientific literature for radiologic evaluation. Radiographic images (CBCT imaging, digital orthopantomography [DOR], and digital periapical radiography) from 55 patients were analyzed. All parameters were evaluated on CBCT, DOR, and digital periapical radiographic images by 2 external observers. The statistical analysis was performed with software SPSS version 19.0 (SPSS Inc, Chicago, IL). Chi-square tests were used to compare frequencies of qualitative variables. The level of significance was set at P ≤ .05. RESULTS: Overall intraobserver and interobserver agreements were very good and good, respectively. CBCT analysis found more lesions and lesions of bigger dimension (P < .001). CBCT imaging was also superior in locating lesions in the apical part on the side compared with DOR and in the diagnosis of cortical bone destruction compared with both methods (P < .001). Through CBCT analysis, more root canals and more canals associated with lesions were found. The most informative and reproducible periapical and endodontic status parameters were selected, and a new PESS was proposed. CONCLUSIONS: The classification proposed in the present study seems to be reproducible and objective and adds helpful information with respect to the existing indexes. Future studies need to be conducted to validate PESS.


Assuntos
Tomografia Computadorizada de Feixe Cônico , Periodontite Periapical/diagnóstico , Radiografia Dentária Digital , Ápice Dentário/diagnóstico por imagem , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Periodontite Periapical/diagnóstico por imagem , Periodontite Periapical/fisiopatologia , Radiografia Panorâmica/métodos , Tratamento do Canal Radicular , Ápice Dentário/fisiopatologia
9.
J Endod ; 41(2): 261-4, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25476974

RESUMO

INTRODUCTION: The aim of this study was to compare the incidence of root cracks after root canal instrumentation with the TF Adaptive, WaveOne, ProTaper Next, and ProTaper Universal systems. METHODS: Seventy-five extracted mandibular central incisors with mature apices and straight root canals (<5°) were selected and kept in distilled water. The root canals were instrumented by using the ProTaper Universal, ProTaper Next, WaveOne, and TF Adaptive systems. All the roots were horizontally sectioned 3, 6, and 9 mm from the apex with a low-speed saw under water cooling. The slices were then viewed through a stereomicroscope at ×25 magnification. The samples were photographed with a camera to determine the presence of dentinal cracks. RESULTS: The control group had no cracks, and the difference between the control group and the experimental groups was statistically significant (P < .001). The ProTaper Next and TF Adaptive systems produced significantly less cracks than the ProTaper Universal and WaveOne systems in the apical section (3 mm) (P < .05). CONCLUSIONS: Under the study conditions and within the limitations of this study, it can be concluded that the ProTaper Universal, ProTaper Next, WaveOne, and TF Adaptive instruments can result in dentinal cracks.


Assuntos
Dentina/fisiopatologia , Incisivo/fisiopatologia , Preparo de Canal Radicular/efeitos adversos , Fraturas dos Dentes/fisiopatologia , Raiz Dentária/fisiopatologia , Instrumentos Odontológicos/efeitos adversos , Cavidade Pulpar/fisiopatologia , Dentina/cirurgia , Humanos , Incisivo/cirurgia , Preparo de Canal Radicular/instrumentação , Ápice Dentário/fisiopatologia , Ápice Dentário/cirurgia , Fraturas dos Dentes/etiologia , Raiz Dentária/cirurgia
10.
J Endod ; 41(2): 253-6, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25433969

RESUMO

INTRODUCTION: The purpose of this study was to investigate the incidence of apical crack initiation and propagation in root dentin after several endodontic procedures. METHODS: Sixty intact mandibular premolars were sectioned perpendicular to the long axis at 1 mm from the apex, and the apical surface was polished. Thirty teeth were left unprepared and served as a control, and the remaining 30 teeth were instrumented with ProTaper Universal instruments (Dentsply Maillefer, Ballaigues, Switzerland) up to size F5. The root canals were filled with the single-cone technique. Gutta-percha was removed with drills of the Rebilda post system (VOCO, Cuxhaven, Germany). Glass fiber-reinforced composite fiber posts were cemented using a dual-cure resin cement. The fiber posts were removed with a drill of the post system. Retreatment was completed after the removal of the gutta-percha. Crack initiation and propagation in the apical surfaces of the samples were examined with a stereomicroscope after each procedure. The absence/presence of cracks was recorded. Logistic regression was performed to analyze statistically the incidence of crack initiation and propagation with each procedure. RESULTS: The initiation of the first crack and crack propagation was associated with F2 and F4 instruments, respectively. The logistic regression analysis revealed that instrumentation and F2 instrument significantly affected apical crack initiation (P < .001). Post space preparation had a significant effect on crack propagation (P = .0004). The other procedures had no significant effects on crack initiation and propagation (P > .05). CONCLUSIONS: Rotary nickel-titanium instrumentation had a significant effect on apical crack initiation, and post space preparation with drills had a significant impact on crack propagation.


Assuntos
Dente Pré-Molar/fisiopatologia , Dentina/fisiopatologia , Ápice Dentário/fisiopatologia , Fraturas dos Dentes/fisiopatologia , Dente Pré-Molar/cirurgia , Guta-Percha/uso terapêutico , Humanos , Mandíbula/fisiopatologia , Mandíbula/cirurgia , Retratamento/métodos , Materiais Restauradores do Canal Radicular/uso terapêutico , Obturação do Canal Radicular , Preparo de Canal Radicular/efeitos adversos , Preparo de Canal Radicular/instrumentação , Hipoclorito de Sódio/uso terapêutico , Ápice Dentário/cirurgia , Fraturas dos Dentes/etiologia , Raiz Dentária/efeitos dos fármacos , Raiz Dentária/fisiopatologia , Raiz Dentária/cirurgia
11.
J Endod ; 41(2): 201-4, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25476973

RESUMO

INTRODUCTION: The aim of this ex vivo study was to evaluate the accuracy of the Raypex 5 (VDW, Munich, Germany) and Apex NRG XFR (Medic NRG Ltd, Tel Aviv, Israel) electronic apex locators (EALs) in determining the working length when compared with radiographs. METHODS: Twenty-five human single-rooted teeth were selected, and the access cavity was prepared. The working length (WL) was determined radiographically and electronically by using 2 EALs. The files were fixed at the WL, and the teeth were extracted. The apical 4 mm of each canal was trimmed to expose the file tip, and the samples were observed under a stereomicroscope. The distance from the file tip to the point 0.5 mm coronal to the anatomic apex was measured. The data were analyzed by using 1-way analysis of variance and the Tukey Honestly Significant Difference test. RESULTS: There was no significant difference between the Raypex 5 and the Apex NRG XFR devices with respect to their accuracy in determining the final WL. When compared with radiography, both the EALs had no significant difference. When comparing EALs and radiographic measurements with control measurements, accuracy results were found to be 20%, 36%, and 52% for the Raypex 5, Apex NRG XFR, and radiography, respectively. Overestimations of WL determination by the Raypex 5, Apex NRG XFR, and radiography were 4%, 0%, and 40%, respectively. Underestimations of WL determination by the Raypex 5, Apex NRG XFR, and radiography were 76%, 64%, and 8%, respectively. CONCLUSIONS: Both the EALs had the same accuracy in determining the WL when compared with radiography.


Assuntos
Instrumentos Odontológicos , Cavidade Pulpar/diagnóstico por imagem , Odontometria/instrumentação , Ápice Dentário/diagnóstico por imagem , Cavidade Pulpar/fisiopatologia , Alemanha , Humanos , Israel , Preparo de Canal Radicular/instrumentação , Ápice Dentário/fisiopatologia
12.
J Endod ; 41(2): 197-200, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25476976

RESUMO

INTRODUCTION: It has been proposed that individual genetic predisposition may contribute to persistent apical periodontitis. Cytokines are associated with levels of inflammation and are involved in caries, pulpal, and periapical tissue destruction. We hypothesized that polymorphisms in cytokine genes may contribute to an individual's increased susceptibility to apical tissue destruction in response to deep carious lesions. METHODS: Subjects with deep carious lesions with or without periapical lesions (≥3 mm) were recruited at the University of Pittsburgh, Pittsburgh, PA, and the University of Texas at Houston, Houston, TX. Genomic DNA samples of 316 patients were sorted into 2 groups: 136 cases with deep carious lesions and periapical lesions (cases) and 180 cases with deep carious lesions but no periapical lesions (controls). Nine single-nucleotide polymorphisms in IL1B, IL6, TNF, RANK, RANKL, and OPG genes were selected for genotyping. Genotypes were generated by end point analysis using TaqMan chemistry (Invitrogen, Carlsbad, CA) in a real-time polymerase chain reaction instrument. Allele and genotype frequencies were compared among cases and controls using the PLINK program (http://pngu.mgh.harvard.edu/purcell/plink/). Ninety-three human periapical granulomas and 24 healthy periodontal ligament tissues collected postoperatively were used for messenger RNA expression analyses of IL1B. RESULTS: A single-nucleotide polymorphism in IL1B (rs1143643) showed allelic (P = .02) and genotypic (P = .004) association with cases of deep caries and periapical lesions. We also observed altered transmission of IL1B marker haplotypes (P = .02) in these individuals. IL1B was highly expressed in granulomas (P < .001). CONCLUSIONS: Variations in IL1B may be associated with periapical lesion formation in individuals with untreated deep carious lesions. Future studies could help predict host susceptibility to developing periapical lesions.


Assuntos
Cárie Dentária/genética , Estudos de Associação Genética , Interleucina-1beta/genética , Periodontite Periapical/genética , Adulto , Idoso , Alelos , Cárie Dentária/fisiopatologia , Feminino , Predisposição Genética para Doença , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Abscesso Periapical/genética , Abscesso Periapical/fisiopatologia , Periodontite Periapical/fisiopatologia , Polimorfismo de Nucleotídeo Único , Ápice Dentário/fisiopatologia
13.
J Endod ; 40(9): 1355-61, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25146015

RESUMO

INTRODUCTION: The purpose of this study was to investigate the effect of conditioned medium (CM) from murine preameloblasts on the cellular differentiation of mesenchymal stem cells (MSCs) in immature teeth with necrotic pulp and apical periodontitis. METHODS: Pulp necrosis and apical periodontitis were induced in 30 immature permanent double-rooted premolars of 3 beagles and were randomly assigned to the following treatment groups: group CM (n = 10), revascularization treatment was performed using CM from preameloblasts of C57BL/6 mice apical bud cells; group CR (n = 10), conventional revascularization treatment was performed; positive control group (n = 5), left infected; and negative control group (n = 5), untreated. The dogs were followed up for 12 weeks and assessed for treatment outcomes with radiographic and histologic analyses. The effect of the CM on sequential Runx2 and osterix messenger RNA gene expression during the differentiation of MG63 osteoblastlike cells was analyzed with real-time polymerase chain reaction. RESULTS: The overall treatment outcomes were not significantly different between the 2 treatment groups. However, the teeth in the CM group showed significantly more mature apices and a higher degree of hard tissue formation with projections intercalating into the pre-existing root dentin (P < .05). In CM-treated teeth, regenerated pulplike tissue was more frequently observed (P < .05). During differentiation, the CM induced early peak expression of Runx2 followed by sustained osterix overexpression. CONCLUSIONS: CM from preameloblasts rendered a favorable effect in providing a physiologic microenvironment for the differentiation of MSCs after revascularization treatment.


Assuntos
Ameloblastos/fisiologia , Necrose da Polpa Dentária/terapia , Periodontite Periapical/terapia , Animais , Antibacterianos/uso terapêutico , Apexificação/métodos , Técnicas de Cultura de Células , Diferenciação Celular/fisiologia , Microambiente Celular/fisiologia , Subunidade alfa 1 de Fator de Ligação ao Core/análise , Meios de Cultivo Condicionados , Cemento Dentário/patologia , Cemento Dentário/fisiopatologia , Polpa Dentária/patologia , Polpa Dentária/fisiopatologia , Necrose da Polpa Dentária/patologia , Necrose da Polpa Dentária/fisiopatologia , Dentina/patologia , Dentina/fisiopatologia , Cães , Células-Tronco Mesenquimais/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Osteoblastos/fisiologia , Periodontite Periapical/patologia , Periodontite Periapical/fisiopatologia , Distribuição Aleatória , Regeneração/fisiologia , Preparo de Canal Radicular/métodos , Fatores de Tempo , Tecidos Suporte , Ápice Dentário/patologia , Ápice Dentário/fisiopatologia , Fatores de Transcrição/análise , Dedos de Zinco
14.
J Endod ; 40(2): 192-8, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24461403

RESUMO

INTRODUCTION: Regenerative endodontics is a promising alternative treatment for immature teeth with necrotic pulps. The present study was performed to assess the regenerative potential of young permanent immature teeth with necrotic pulp after the following treatment protocols: (1) a mineral trioxide aggregate (MTA) apical plug, (2) the regenerative endodontic protocol (blood clot scaffold), and (3) the regenerative endodontic protocol with a blood clot and an injectable scaffold impregnated with basic fibroblast growth factor. METHODS: Immature necrotic permanent maxillary central incisors (n = 36) of patients 9-13 years old were divided into 3 groups according to the treatment protocol: the MTA group (MTA apical plug), the REG group (regenerative endodontic protocol [blood clot]), and the FGF group (regenerative endodontic protocol [blood clot + injectable scaffold]). Follow-up was done up to 18 months. Standardized radiographs were digitally evaluated for an increase in root length and thickness, a decrease in the apical diameter, and a change in periapical bone density. RESULTS: After a follow-up period of 18 months, most of the cases showed radiographic evidence of periapical healing. Groups 2 and 3 showed a progressive increase in root length and width and a decrease in apical diameter. CONCLUSIONS: The regenerative endodontic procedure allowed the continued development of roots in teeth with necrotic pulps. The use of artificial hydrogel scaffold and basic fibroblast growth factor was not essential for repair.


Assuntos
Compostos de Alumínio/uso terapêutico , Apexificação/métodos , Compostos de Cálcio/uso terapêutico , Necrose da Polpa Dentária/terapia , Óxidos/uso terapêutico , Regeneração/fisiologia , Materiais Restauradores do Canal Radicular/uso terapêutico , Silicatos/uso terapêutico , Engenharia Tecidual/métodos , Adolescente , Antibacterianos/administração & dosagem , Coagulação Sanguínea/fisiologia , Densidade Óssea/fisiologia , Criança , Ciprofloxacina/administração & dosagem , Doxiciclina/administração & dosagem , Combinação de Medicamentos , Feminino , Fator 2 de Crescimento de Fibroblastos/uso terapêutico , Seguimentos , Humanos , Masculino , Metronidazol/administração & dosagem , Tecido Periapical/fisiopatologia , Radiografia Interproximal/métodos , Radiografia Dentária Digital/métodos , Tecidos Suporte , Ápice Dentário/fisiopatologia , Raiz Dentária/fisiopatologia
15.
Dental Press J Orthod ; 18(4): 104-12, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24262423

RESUMO

OBJECTIVE: To evaluate the frequency of Apical Root Resorption (ARR) after orthodontic treatment at 52-288 months using periapical radiography (PR) and cone beam computed tomography (CBCT). METHODS: Radiographic images obtained from 58 patients, before (T1) and after orthodontic treatment (T2), and following 52-288 months of treatment were analyzed by three members of the Brazilian Board of Orthodontics. Apical structures were evaluated by PR images (T2 and T3), using Levander and Malmgren scores. The presence of ARR on CBCT images was detected only at T3. The Kolmogorov-Smirnov test was used for statistical analyses, and the level of significance was set at 5%. Kappa statistics determined interobserver agreement. RESULTS: The more frequent ARR were with scores 1 in T2 (51.6%) and T3 (53.1%), when evaluated by PR (p > 0.05). When compared the frequencies of ARR in T3 among PR and CBCT images, the differences were significant for maxillary and mandibular premolar groups, and for mandibular molar group (p > 0.05). The teeth with highest frequency of ARR presence using CBCT images were maxillary lateral incisors (94.5%) and mandibular central incisors (87.7%), while the premolars showed the lowest frequency. The CBCT images showed that the teeth involved in orthodontic treatment with extraction present higher ARR frequency (p < 0.05). CONCLUSIONS: PR showed more frequency of ARR in posterior teeth groups when compared with CBCT images. ARR did not change in long-term post treatment.


Assuntos
Tomografia Computadorizada de Feixe Cônico/métodos , Ortodontia Corretiva/efeitos adversos , Radiografia Dentária/métodos , Reabsorção da Raiz/etiologia , Ápice Dentário/patologia , Ápice Dentário/fisiopatologia , Adolescente , Criança , Tomografia Computadorizada de Feixe Cônico/instrumentação , Feminino , Humanos , Radiografia Dentária/instrumentação , Estudos Retrospectivos , Reabsorção da Raiz/diagnóstico por imagem , Índice de Gravidade de Doença , Fatores de Tempo , Ápice Dentário/diagnóstico por imagem
16.
Claves odontol ; 20(71): 22-28, nov. 2013. ilus
Artigo em Espanhol | LILACS | ID: lil-719599

RESUMO

En el presente artículo se describe un caso clínico de incisivos afectados por reabsorción apical externa. Dado que el tratamiento de la reabsorción requiere la aplicación de numerosas maniobras apropiadas, se comenzó por realizar el diagnóstico clínico-radiográfico adecuado. El paciente presentaba ortodoncia fija superior e inferior, edema en el fondo de surco de la zona anteroinferior, test de vitalidad negativo en los dientes 31 y 41, incisivos inferiores asintomáticos y con respuesta positiva a las pruebas de vitalidad. Concluido el diagnóstico, se aplicó un procedimiento alternativo que consistió en la inmediata supresión de las fuerzas ortodónticas, aperturas camerales en las piezas 31 y 41, medicación y cronificación del proceso agudo. Con el fin de lograr la reparación de las piezas afectadas, estos dientes fueron tratados con sesiones intermedias de pasta alcalina, compuesta por hidróxido de calcio, iodoformo y clorofenol alcanforado. Al observar en los controles silencio clínico y comienzo de neoformación ósea en la zona, se realizó la terapia definitiva. Los resultados obtenidos muestran que si bien los tratamientos de dientes con reabsorción apical externa son intervenciones complicadas, son posibles de tratar sin necesidad de recurrir a intervenciones quirúrgicas, aportando beneficios psicológicos y económicos para el paciente.


Assuntos
Humanos , Masculino , Adolescente , Ápice Dentário/fisiopatologia , Materiais Restauradores do Canal Radicular/química , Reabsorção da Raiz/etiologia , Tratamento do Canal Radicular/efeitos adversos , Hidróxido de Cálcio , Cânfora , Compostos de Iodo , Osteogênese/fisiologia
17.
Claves odontol ; 20(71): 22-28, nov. 2013. ilus
Artigo em Espanhol | BINACIS | ID: bin-130056

RESUMO

En el presente artículo se describe un caso clínico de incisivos afectados por reabsorción apical externa. Dado que el tratamiento de la reabsorción requiere la aplicación de numerosas maniobras apropiadas, se comenzó por realizar el diagnóstico clínico-radiográfico adecuado. El paciente presentaba ortodoncia fija superior e inferior, edema en el fondo de surco de la zona anteroinferior, test de vitalidad negativo en los dientes 31 y 41, incisivos inferiores asintomáticos y con respuesta positiva a las pruebas de vitalidad. Concluido el diagnóstico, se aplicó un procedimiento alternativo que consistió en la inmediata supresión de las fuerzas ortodónticas, aperturas camerales en las piezas 31 y 41, medicación y cronificación del proceso agudo. Con el fin de lograr la reparación de las piezas afectadas, estos dientes fueron tratados con sesiones intermedias de pasta alcalina, compuesta por hidróxido de calcio, iodoformo y clorofenol alcanforado. Al observar en los controles silencio clínico y comienzo de neoformación ósea en la zona, se realizó la terapia definitiva. Los resultados obtenidos muestran que si bien los tratamientos de dientes con reabsorción apical externa son intervenciones complicadas, son posibles de tratar sin necesidad de recurrir a intervenciones quirúrgicas, aportando beneficios psicológicos y económicos para el paciente.(AU)


Assuntos
Humanos , Masculino , Adolescente , Reabsorção da Raiz/etiologia , Tratamento do Canal Radicular/efeitos adversos , Materiais Restauradores do Canal Radicular/química , Ápice Dentário/fisiopatologia , Osteogênese/fisiologia , Cânfora , Hidróxido de Cálcio , Compostos de Iodo
18.
J Prosthet Dent ; 110(4): 303-12, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24079566

RESUMO

STATEMENT OF PROBLEM: Periodontally compromised teeth (PCT) that serve as terminal abutments (TAs) are often challenging depending on the post-and-core treatment, the type of partial fixed dental prosthesis (PFDP), and the periodontal support. PURPOSE: The purpose of this study was to investigate the biomechanical impact of 3 types of PFDP supported by cast post-and-cores on PCT serving as terminal abutments. MATERIAL AND METHODS: A 3-dimensional (3D) model of a human mandible was fabricated by using computed tomography (CT) images and parameterized in a computer-aided design (CAD) environment as follows: Right premolar preparation geometries were designed. The second premolar was assembled with 7-mm or 10-mm cast post-and-core models. Both premolar-models were designed to support single, splinted, or 1-unit cantilever splinted crowns. In each situation, their periodontium geometries were designed to be reduced by 10%, 50%, and 70%. All models were imported into a 3D finite element analysis (FEA) environment and loaded; von Mises stress values and distribution patterns were evaluated. RESULTS: Insertion of the post primarily affected the apical areas of both the root and post; the type of PFDP and periodontal support mainly affected stress distribution. In patients with a normal periodontium, splinting the teeth did not contribute to their stress relief. By extending the post length, a stressful area close to the apex of the post was developed. Splinting mitigated the stress field of the coronal part of the 50% PCT (up to 98.9%); the 30% PCT experienced a substantial decrease (up to 215.9%) in stress in the radical part as well. The increase in the length of the post produced negligible stress-related differences in the apical part of the 50% PCT (0.2% to 2.6%). The use of the 7-mm post effectively relieved the radical part of the splinted 30% PCT. The magnitude of the stress on the radical part of post-restored PCT was considerably increased in the presence of a cantilever. CONCLUSIONS: Splinted crowns supported by a 7-mm cast post-and-core are a favorable biomechanical approach for the restoration of PCT with severe loss of coronal structure. The use of a cantilever greatly aggravates the biomechanical response, especially of post-restored PCT.


Assuntos
Dente Suporte , Planejamento de Dentadura , Prótese Parcial Fixa , Doenças Periodontais/fisiopatologia , Técnica para Retentor Intrarradicular , Processo Alveolar/fisiopatologia , Dente Pré-Molar/fisiopatologia , Fenômenos Biomecânicos , Força de Mordida , Desenho Assistido por Computador , Coroas , Dentina/fisiopatologia , Módulo de Elasticidade , Análise de Elementos Finitos , Humanos , Processamento de Imagem Assistida por Computador/métodos , Imageamento Tridimensional/métodos , Mandíbula/anatomia & histologia , Teste de Materiais , Modelos Anatômicos , Modelos Biológicos , Técnica para Retentor Intrarradicular/instrumentação , Estresse Mecânico , Tomografia Computadorizada por Raios X/métodos , Ápice Dentário/fisiopatologia , Raiz Dentária/fisiopatologia
19.
Dental press j. orthod. (Impr.) ; 18(4): 104-112, July-Aug. 2013. ilus, tab
Artigo em Inglês | LILACS | ID: lil-695126

RESUMO

OBJECTIVE: To evaluate the frequency of Apical Root Resorption (ARR) after orthodontic treatment at 52-288 months using periapical radiography (PR) and cone beam computed tomography (CBCT). METHODS: Radiographic images obtained from 58 patients, before (T1) and after orthodontic treatment (T2), and following 52-288 months of treatment were analyzed by three members of the Brazilian Board of Orthodontics. Apical structures were evaluated by PR images (T2 and T3), using Levander and Malmgren scores. The presence of ARR on CBCT images were detected only at T3. The Kolmogorov-Smirnov test was used for statistical analyses, and the level of significance was set at 5%. Kappa statistics determined interobserver agreement. RESULTS: The more frequent ARR were with scores 1 in T2 (51.6%) and T3 (53.1%), when evaluated by PR (p > 0.05). When compared the frequencies of ARR in T3 among PR and CBCT images, the differences were significant for maxillary and mandibular pre-molar groups, and for mandibular molar group (p > 0.05). The teeth with highest frequency of ARR presence using CBCT images were maxillary lateral incisors (94.5%) and mandibular central incisors (87.7%), while the premolars showed the lowest frequency. The CBCT images showed that the teeth involved in orthodontic treatment with extraction present higher ARR frequency (p < 0.05). CONCLUSION: PR showed more frequency of ARR in posterior teeth groups when compared with CBCT images. ARR did not change in long-term post treatment.


OBJETIVO: avaliar a frequência de reabsorção radicular apical (RRA) após tratamento ortodôntico, em longo prazo, por meio de imagens de radiografia periapical (RP) e tomografia computadorizada de feixe cônico (TCFC). MÉTODOS: as imagens radiográficas obtidas de dentes de 58 pacientes, antes (T1), após o tratamento ortodôntico (T2) e decorridos pelo menos 52 meses do tratamento (T3), foram analisadas por três examinadores, membros do Board Brasileiro de Ortodontia e Ortopedia Facial. As estruturas apicais foram avaliadas por meio de imagens de RP (T2 e T3), utilizando o sistema de escores de Levander e Malmgren modificado. A presença de RRA nas imagens tomográficas obtidas em T3 foi detectada por um especialista em radiologia com experiência em TCFC. Os dados foram estatisticamente analisados pelo teste de Kolmogorov-Smirnov, com nível de significância de 5%. O teste kappa determinou o nível de concordância entre os observadores. RESULTADOS: as RRAs mais frequentes foram as de escore 1 em T2 (51,6%) e em T3 (53,1%), quando avaliadas por RPs (p > 0,05). Quando comparadas as frequências de RRAs em T3, entre imagens de RP e de TCFC, a diferença foi estatisticamente significativa para o grupo de pré-molares da maxila e da mandíbula, e de molares mandibulares. Os dentes que apresentaram maior frequência de RRA quando analisados por meio de imagens de TCFC, foram os incisivos laterais superiores (94,5%) e os centrais inferiores (87,7%), enquanto os de menor frequência foram os pré-molares. As imagens de TCFC mostraram que os dentes envolvidos em tratamentos ortodônticos com extrações apresentaram maior frequência de RRA (p < 0,05). CONCLUSÃO: as radiografias periapicais mostraram maior frequência de RRAs que as imagens de TCFC para os grupos de pré-molares e molares, não evidenciando alteração em longo prazo.


Assuntos
Adolescente , Criança , Feminino , Humanos , Tomografia Computadorizada de Feixe Cônico/métodos , Ortodontia Corretiva/efeitos adversos , Radiografia Dentária/métodos , Reabsorção da Raiz/etiologia , Ápice Dentário/patologia , Ápice Dentário/fisiopatologia , Tomografia Computadorizada de Feixe Cônico/instrumentação , Estudos Retrospectivos , Radiografia Dentária/instrumentação , Reabsorção da Raiz , Índice de Gravidade de Doença , Fatores de Tempo , Ápice Dentário
20.
Indian J Dent Res ; 24(1): 93-7, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23852240

RESUMO

OBJECTIVE: To analyze the effect of different combinations of post and core materials on stress distribution in dentin of an endodontically treated tooth. MATERIALS AND METHODS: This was an experimental stress analysis study. Models were made in photoelastic material, i.e., epoxy resin. Different combinations of post and core materials used were: Glass fiber post with composite core, stainless steel post with composite core, and cast metal post and core. Stresses generated were frozen, models were sliced and viewed under circular polariscope, and photographs were taken. Stress was calculated by counting the number of fringes. RESULTS: For the combination of glass fiber post with composite core, the shear stresses calculated were 1.196, 1.196, and 2.898 MPa in the apical, mid-root, and cervical region, respectively. For the combination of stainless steel post with composite core, the apical, mid-root and cervical stresses were 1.534, 0.511, and 2.557 MPa, respectively. For cast metal post and core, the apical, mid-root, and cervical stresses were 0.852, 0.511, and 1.534 MPa, respectively. CONCLUSION: The cervical region of the teeth is subjected to the highest stresses irrespective of the material used. The stainless steel post with the composite core generated the highest stress concentration in different regions. A glass fiber post generated a uniform stress distribution. A cast metal post and core combination generated lesser stress than the other combinations. The vast difference in the elastic modulus of the restorative materials can lead to nonuniform stress distribution and concentration of stresses in different areas which can have deleterious effect on the survival of already compromised teeth and restoration. Such combinations should be avoided and the material which has an elastic modulus close to that of dentin should be preferred.


Assuntos
Materiais Dentários/química , Dentina/fisiopatologia , Técnica para Retentor Intrarradicular/instrumentação , Fenômenos Biomecânicos , Ligas de Cromo/química , Resinas Compostas/química , Ligas Dentárias/química , Cavidade Pulpar/fisiopatologia , Módulo de Elasticidade , Resinas Epóxi/química , Vidro/química , Humanos , Fotografação , Anidridos Ftálicos/química , Polivinil/química , Siloxanas/química , Aço Inoxidável/química , Estresse Mecânico , Ápice Dentário/fisiopatologia , Colo do Dente/fisiopatologia , Dente não Vital/fisiopatologia
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